The role of hepatic mitochondria in the regulation of glucose metabolism in BHE rats

Miscellaneous · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7255382

Kim, M J.C.

The interacting effects of dietary fat source and thyroxine treatment on the hepatic mitochondrial function and glucose metabolism were studied. In the first study, three different sources of dietary fatty acids and thyroxine treatment were used to investigate the hepatic mitochondrial thermotropic behavior in two strains of rat. The NIDDM BHE and Sprague-Dawley rats were used. Feeding coconut oil increased serum T{sub 4} levels and T{sub 4} treatment increased serum T{sub 3} levels in the BHE rats. In the mitochondria from BHE rats fed coconut oil and treated with T{sub 4}, the transition temperature disappeared due to a decoupling of succinate supported respiration. This was not observed in the Sprague-Dawley rats. In the second study, two different sources of dietary fat and T{sub 4} treatment were used to investigate hepatic mitochondrial function. Coconut oil feeding increased Ca{sup ++}Mg{sup ++}ATPase and Mg{sup ++}ATPase. T{sub 4} treatment had potentiated this effect. T{sub 4} increased the malate-aspartate shuttle and {alpha}-glycerophosphate shuttle activities. In the third study, the glucose turnover rate from D-({sup 14}C-U)/(6-{sup 3}H)-glucose and gluconeogeneis from L-({sup 14}C-U)-alanine was examined. Dietary fat or T{sub 4} did not affect the glucose mass. T{sub 4} increased the irreversible fractional glucose turnover rate.

• Research Organization: Georgia Univ., Athens, GA (USA)
• OSTI ID: 7255382
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
BIOLOGICAL EFFECTS
GLUCOSE
METABOLISM
MITOCHONDRIA
BIOLOGICAL FUNCTIONS
THYROXINE
ALANINES
ATP-ASE
CARBON 14 COMPOUNDS
DIET
GENETIC VARIABILITY
LIVER
RATS
TRACER TECHNIQUES
TRIIODOTHYRONINE
ACID ANHYDRASES
ALDEHYDES
AMINO ACIDS
ANIMALS
BIOLOGICAL VARIABILITY
BODY
CARBOHYDRATES
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
FUNCTIONS
GLANDS
HEXOSES
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PHOSPHOHYDROLASES
RODENTS
SACCHARIDES
THYROID HORMONES
VERTEBRATES
550501* - Metabolism- Tracer Techniques