CArG boxes in the human cardiac. cap alpha. -actin gene are core binding sites for positive trans-acting regulatory factors

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.19.6702· OSTI ID:7253515

Miwa, T; Boxer, L M; Kedes, L

Positively acting, rate-limiting regulatory factors that influence tissue-specific expression of the human cardiac ..cap alpha..-actin gene in a mouse muscle cell line are shown by in vivo competition and gel mobility-shift assays to bind to upstream regions of its promoter but to neither vector DNA not a ..beta..-globin promoter. Although the two binding regions are distinctly separated, each corresponds to a cis region required for muscle-specific transcriptional stimulation, and each contains a core CC(A+T-rich)/sub 6/GC sequence (designated CArG box), which is found in the promoter regions of several muscle-associated genes. Each site has an apparently different binding affinity for trans-acting factors, which may explain the different transcriptional stimulation activities of the two cis regions. Therefore, the authors conclude that the two CArG box regions are responsible for muscle-specific transcriptional activity of the cardiac ..cap alpha..-actin gene through a mechanism that involves their binding of a positive trans-acting factor in muscle cells.

• Research Organization: Stanford Univ. School of Medicine, Palo Alto, CA (USA)
• OSTI ID: 7253515
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:19
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE REPRESSORS
DNA SEQUENCING
GENE REGULATION
GENES
TRANSCRIPTION
ACTIN
HEART
IN VIVO
MAN
MICE
MUSCLES
PHOSPHORUS 32
TRANSFERASES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
ENZYMES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques