skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. [Spinacea oleracea; Triticum aestivum; Arabidopsis thaliana; Maize; Phaseolus vulgaris; Petunia hybrida]

Abstract

The level of 2-carboxyarabinitol 1-phosphate (CA1P) in leaves of 12 species was determined by an isotope dilution assay. {sup 14}C-labeled standard was synthesized from (2-{sup 14}C)carboxyarabinitol 1,5-bisphosphate using acid phosphatase, and was added at the initial point of leaf extraction. Leaf CA1P was purified and its specific activity determined. CA1P was found in dark-treated leaves of all species examined, including spinach (Spinacea oleracea), wheat (Triticum aestivum), Arabidopsis thaliana, and maize (Zea mays). The highest amounts were found in bean (Phaseolus vulgaris) and petunia (Petunia hybrida), which had 1.5 to 1.8 moles CA1P per mole ribulose 1,5-bisphosphate carboxylase catalytic sites. Most species had intermediate amounts of CA1P (0.2 to 0.8 mole CA1P per mole catalytic sites). Such intermediate to high levels of CA1P support the hypothesis that CA1P functions in many species as a light-dependent regulator of ribulose 1,5-bisphosphate carboxylase activity and whole leaf photosynthetic CO{sub 2} assimilation. However, CA1P levels in spinach, wheat, and A. thaliana were particularly low (less than 0.09 mole CA1P per mole catalytic sites). In such species, CA1P does not likely have a significant role in regulating ribulose 1,5-bisphosphate carboxylase activity, but could have a different physiological role.

Authors:
; ;  [1]
  1. Univ. of Nevada, Reno (United States)
Publication Date:
OSTI Identifier:
7250062
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (United States)
Additional Journal Information:
Journal Volume: 96:1; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CROPS; PHYSIOLOGY; ENZYME INHIBITORS; MEASURING METHODS; ORNAMENTAL PLANTS; ACID PHOSPHATASE; ARABIDOPSIS; CARBON 14 COMPOUNDS; ENZYME ACTIVITY; ISOTOPE DILUTION; LEAVES; MAIZE; PHOTOSYNTHESIS; PLANTS; RIBULOSE DIPHOSPHATE CARBOXYLASE; SPINACH; WHEAT; CARBON COMPOUNDS; CARBON-CARBON LYASES; CARBOXY-LYASES; CEREALS; CHEMICAL REACTIONS; ENZYMES; ESTERASES; FOOD; GRAMINEAE; HYDROLASES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LILIOPSIDA; LYASES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; ORGANIC COMPOUNDS; PHOSPHATASES; PHOTOCHEMICAL REACTIONS; PROTEINS; SYNTHESIS; TRACER TECHNIQUES; VEGETABLES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Moore, B D, Kobza, J, and Seemann, J R. Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. [Spinacea oleracea; Triticum aestivum; Arabidopsis thaliana; Maize; Phaseolus vulgaris; Petunia hybrida]. United States: N. p., 1991. Web. doi:10.1104/pp.96.1.208.
Moore, B D, Kobza, J, & Seemann, J R. Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. [Spinacea oleracea; Triticum aestivum; Arabidopsis thaliana; Maize; Phaseolus vulgaris; Petunia hybrida]. United States. https://doi.org/10.1104/pp.96.1.208
Moore, B D, Kobza, J, and Seemann, J R. 1991. "Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. [Spinacea oleracea; Triticum aestivum; Arabidopsis thaliana; Maize; Phaseolus vulgaris; Petunia hybrida]". United States. https://doi.org/10.1104/pp.96.1.208.
@article{osti_7250062,
title = {Measurement of 2-carboxyarabinitol 1-phosphate in plant leaves by isotope dilution. [Spinacea oleracea; Triticum aestivum; Arabidopsis thaliana; Maize; Phaseolus vulgaris; Petunia hybrida]},
author = {Moore, B D and Kobza, J and Seemann, J R},
abstractNote = {The level of 2-carboxyarabinitol 1-phosphate (CA1P) in leaves of 12 species was determined by an isotope dilution assay. {sup 14}C-labeled standard was synthesized from (2-{sup 14}C)carboxyarabinitol 1,5-bisphosphate using acid phosphatase, and was added at the initial point of leaf extraction. Leaf CA1P was purified and its specific activity determined. CA1P was found in dark-treated leaves of all species examined, including spinach (Spinacea oleracea), wheat (Triticum aestivum), Arabidopsis thaliana, and maize (Zea mays). The highest amounts were found in bean (Phaseolus vulgaris) and petunia (Petunia hybrida), which had 1.5 to 1.8 moles CA1P per mole ribulose 1,5-bisphosphate carboxylase catalytic sites. Most species had intermediate amounts of CA1P (0.2 to 0.8 mole CA1P per mole catalytic sites). Such intermediate to high levels of CA1P support the hypothesis that CA1P functions in many species as a light-dependent regulator of ribulose 1,5-bisphosphate carboxylase activity and whole leaf photosynthetic CO{sub 2} assimilation. However, CA1P levels in spinach, wheat, and A. thaliana were particularly low (less than 0.09 mole CA1P per mole catalytic sites). In such species, CA1P does not likely have a significant role in regulating ribulose 1,5-bisphosphate carboxylase activity, but could have a different physiological role.},
doi = {10.1104/pp.96.1.208},
url = {https://www.osti.gov/biblio/7250062}, journal = {Plant Physiology; (United States)},
issn = {0032-0889},
number = ,
volume = 96:1,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}