Preparation of a cDNA library from the hagfish slime gland

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7246485

Gadbois, D M; Salo, W L; Ann, D K; Downing, S W; Carlson, D M

The slime glands of hagfish have two major cell types, gland thread cells (GTCs) and gland mucous cells (GMCs), both of which upon contact with water contribute to an abundant viscous mucus. GTCs are unique specialized cells in which 60-80% of the mature GTC volume is occupied by a single tapered thread about 60 cm long formed by bundling keratin intermediate filaments (IFs). Each IF is composed of three 63.5 kD protein subunits (..cap alpha.., ..beta.., ..gamma..). Poly (A/sup +/) mRNA was isolated from GTCs and translated by the rabbit reticulocyte system with (/sup 35/S) Met. Analysis of the translation products showed a major labeled protein at 65 kD. A cDNA library prepared from the poly (A/sup +/) mRNAs was screened with /sup 32/P-labeled IF probes from human epidermis. Several clones containing cross-hybridizing inserts were identified.

• Research Organization: Univ. of Minnesota, Duluth
• OSTI ID: 7246485
• Report Number(s): CONF-8606151-; TRN: 86-039102
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
DNA-CLONING
EEL
GLANDS
CLONE CELLS
MESSENGER-RNA
PHOSPHORUS 32
PROTEINS
SULFUR 35
TRACER TECHNIQUES
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CULTURES
CLONING
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FISHES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques