Site of origin and mechanism of action of adenosine in the frog sympathetic ganglion

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:7244622

Bencherif, M

The contribution of pre and postsynaptic activation on the release of {sup 3}H-purines was studied in the isolated sympathetic paravertebral ganglion of the frog. Preganglionic stimulation induced an overall release of {sup 3}H-purines. This release is blocked by atropine and curare and can be induced by carbachol and antidromic stimulation. Analyses of the effluent by anion exchange chromatography and by HPLC showed that the non-nucleotide fractions constituted most of the counts released. Hence, nucleosides are the main products released by the ganglion and did not arise from hydrolysis of extracellular ATP. We studied the effect of synaptic activity on tritiated inositol release (IR). This release did not change during orthodromic stimulation. However, upon cessation of the stimulation, release increased rapidly and remained elevated for at least 45 minutes. This increase in IR was reduced by suffusion of the ganglia with either acetylcholine or adenosine.

• Research Organization: Virginia Univ., Charlottesville, VA (USA)
• OSTI ID: 7244622
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
BIOCHEMICAL REACTION KINETICS
NERVE CELLS
BIOCHEMISTRY
FROGS
INOSITOL
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
PURINES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
CARBOHYDRATES
CHEMISTRY
CHROMATOGRAPHY
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
MONOSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques