Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers

Crielaard, W; Driessen, A J; Molenaar, D; Hellingwerf, K J; Konings, W N

Title: Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers

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Abstract

Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I pigment protein complexes were fused with membrane vesicles of Streptococcus cremoris or Clostridium acetobutylicum by freeze-thawing and sonication. Illumination of these fused membranes resulted in the generation of a proton motive force of approximately -110 mV. The magnitude of the proton motive force in these membranes could be varied by changing the light intensity. As a result of this proton motive force, amino acid transport into the fused membranes could be observed. The initial rate of leucine transport by membrane vesicles of S. cremoris increased exponentially with the proton motive force. An H+/leucine stoichiometry of 0.8 was determined from the steady-state level of leucine accumulation and the proton motive force, and this stoichiometry was found to be independent of the magnitude of the proton motive force. These results indicate that the introduction of bacterial reaction centers in membrane vesicles by the fusion procedure yields very attractive model systems for the study of proton motive force-consuming processes in membrane vesicles of (strict) anaerobic bacteria.

Authors:: Crielaard, W; Driessen, A J; Molenaar, D; Hellingwerf, K J; Konings, W N

Publication Date:: Fri Apr 01 00:00:00 EST 1988

Research Org.:: Univ. of Groningen, Haren (Netherlands)

OSTI Identifier:: 7242476

Resource Type:: Journal Article

Journal Name:: J. Bacteriol.; (United States)

Additional Journal Information:: Journal Volume: 170:4

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; AMINO ACIDS; MEMBRANE TRANSPORT; CLOSTRIDIUM; PHOTOSYNTHETIC REACTION CENTERS; STREPTOCOCCUS; CELL MEMBRANES; LEUCINE; LIPOSOMES; PHOTOSYNTHETIC MEMBRANES; PROTONS; STOICHIOMETRY; BACTERIA; BARYONS; CARBOXYLIC ACIDS; CELL CONSTITUENTS; ELEMENTARY PARTICLES; FERMIONS; HADRONS; MEMBRANES; MICROORGANISMS; NUCLEONS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANOIDS; 550200* - Biochemistry

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                    Crielaard, W, Driessen, A J, Molenaar, D, Hellingwerf, K J, and Konings, W N. Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers.  United States: N. p., 1988. 
        Web.

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                    Crielaard, W, Driessen, A J, Molenaar, D, Hellingwerf, K J, & Konings, W N. Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers.  United States.

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                    Crielaard, W, Driessen, A J, Molenaar, D, Hellingwerf, K J, and Konings, W N. 1988.  
        "Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers".  United States.

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@article{osti_7242476,

  title        = {Light-driven amino acid uptake in Streptococcus cremoris or Clostridium acetobutylicum membrane vesicles fused with liposomes containing bacterial reaction centers},

  author       = {Crielaard, W and Driessen, A J and Molenaar, D and Hellingwerf, K J and Konings, W N},

  abstractNote = {Reaction centers of the phototrophic bacterium Rhodopseudomonas palustris were introduced as proton motive force-generating systems in membrane vesicles of two anaerobic bacteria. Liposomes containing reaction center-light-harvesting complex I pigment protein complexes were fused with membrane vesicles of Streptococcus cremoris or Clostridium acetobutylicum by freeze-thawing and sonication. Illumination of these fused membranes resulted in the generation of a proton motive force of approximately -110 mV. The magnitude of the proton motive force in these membranes could be varied by changing the light intensity. As a result of this proton motive force, amino acid transport into the fused membranes could be observed. The initial rate of leucine transport by membrane vesicles of S. cremoris increased exponentially with the proton motive force. An H+/leucine stoichiometry of 0.8 was determined from the steady-state level of leucine accumulation and the proton motive force, and this stoichiometry was found to be independent of the magnitude of the proton motive force. These results indicate that the introduction of bacterial reaction centers in membrane vesicles by the fusion procedure yields very attractive model systems for the study of proton motive force-consuming processes in membrane vesicles of (strict) anaerobic bacteria.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/7242476},
  journal      = {J. Bacteriol.; (United States)},
number       = ,

  volume       = 170:4,

  place        = {United States},

  year         = {Fri Apr 01 00:00:00 EST 1988},

  month        = {Fri Apr 01 00:00:00 EST 1988}

}

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