Binding of aluminum to human serum transferrin, human serum albumin and rat serum proteins

Journal Article · Sat Jan 01 00:00:00 EST 1994 · Journal of Environmental Science and Health, Part B, Pesticides, Food Contaminants, and Agricultural Wastes; (United States)

DOI:https://doi.org/10.1080/03601239409372882· OSTI ID:7239289

El-Sebae, A K.H.; Zeid, M M.A.; Abdel-Rahman, F H; Saleh, M A ^[1]

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1. Texas Southern Univ., Houston, TX (United States). Environmental Chemistry and Toxicology Lab.

Human serum transferrin (HSTF), human serum albumin (HSA) and rat serum were compared for their interaction with AlCl[sub 3], in a Tris-HCl buffer solutions. The AlCl[sub 3] was tested in series of concentrations in the range of 50 [mu]M up to 500 [mu]M. HSTF, HSA and their 1:1 mixture and rat serum were incubated at 37 C with series of AlCl[sub 3] concentrations. The protein profile of the incubated solutions were compared to control using SDS-PAGE and FPLC tests. The results indicated that HSTF was more specifically responsive to AlCl[sub 3] showing a characteristic increase in it UV absorption, peak and area dimensions. Simultaneously, HSA was less affected, but it showed a significant shift with an increase in molecular weight accompanied with a change in its profile. The respective bands of transferrin and albumin in rat serum behaved similarly.

• OSTI ID: 7239289
• Journal Information: Journal of Environmental Science and Health, Part B, Pesticides, Food Contaminants, and Agricultural Wastes; (United States), Vol. 29:2; ISSN 0360-1234
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM
METABOLISM
PROTEINS
BIOCHEMICAL REACTION KINETICS
ALBUMINS
ELECTROPHORESIS
TRANSFERRIN
ELEMENTS
GLOBULINS
GLOBULINS-BETA
KINETICS
METALLOPROTEINS
METALS
ORGANIC COMPOUNDS
REACTION KINETICS
560300* - Chemicals Metabolism & Toxicology