Fungal metabolism and detoxification of fluoranthene. [Cunninghamella elegans]

Journal Article · Sun Mar 01 00:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:7235724

Pothuluri, J V; Heflich, R H; Fu, P P; Cerniglia, C E ^[1]

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1. Food and Drug Administration, Jefferson, AR (United States)

Five metabolites produced by Cunninghamella elegans from fluoranthene (FA) in biotransformation studies were investigated for mutagenic activity towards Salmonella typhimurium TA100 and TA104. Whereas FA displayed positive, dose-related mutagenic responses in both tester strains in the presence of a rat liver homogenate fraction, 3-FA-{beta}-glucopyranoside, 3-(8-hydroxy-FA)-{beta}-glucopyranoside, FA trans-2,3-dihydrodiol, and 8-hydroxy-FA trans-2,3-dihydrodiol were negative. 9-Hydroxy-FA trans-2,3-dihydrodiol showed a weak positive response in S. typhimurium TA100. Mutagenicity assays performed with samples extracted at 24-h intervals during incubation of C. elegans with FA for 120 h showed that mutagenic activity decreased with time. Comparative studies with rat liver microsomes indicated that FA trans-2,3-dihydrodiol, the previously identified proximal mutagenic metabolite of FA, was the major metabolite. The circular dichroism spectrum of the rat liver microsomal FA trans-2,3-dihydrodiol indicated that is was optically active. In contrast, the circular dichroism spectrum of the fungal FA trans-2,3-dihydrodiol showed no optical activity. These results indicate that C. elegans has the potential to detoxify FA and that the stereochemistry of its trans-2,3-dihydrodiol metabolite reduces its mutagenic potential.

• OSTI ID: 7235724
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 58:3; ISSN 0099-2240
• Country of Publication: United States
• Language: English

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