Fibrillin binds calcium and is coded by cDNAs that reveal a multidomain structure and alternatively spliced exons at the 5[prime] end
- Oregon Health Sciences Univ., Portland (United States)
- Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States)
Fibrillin is an important structural protein of the extracellular matrix. It is a large cysteine-rich glycoprotein with extensive intrachain disulfide bonds, likely contributed by multiple EGF-like repeats. The authors have previously published 6.9 kb of FBN1 cDNA sequence. FBN1 cDNA clones that extend the sequence 3089 bp in the 5[prime] direction are described in this report. The deduced primary structure suggests that fibrillin in composed of multiple domains. The most predominant features the presence of 43 calcium binding EGF-like repeats. They demonstrate here that fibrillin molecules bind calcium. In addition, three alternatively spliced exons at the 5[prime] end are described. Analysis of 5.8 kb of surrounding genomic sequence revealed a 1.8-kb CpG island spanning the alternatively spliced exons and the next downstream exon. Since FBN1 is the gene responsible for Marfan syndrome, the information presented here will be useful in identifying new mutations and in understanding the function of fibrillin in the pathogenesis of the disease. 42 refs., 7 figs.
- OSTI ID:
- 7225719
- Journal Information:
- Genomics; (United States), Vol. 17:2; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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CARDIOVASCULAR DISEASES
PATHOGENESIS
EYES
HEREDITARY DISEASES
GENES
DNA SEQUENCING
GLYCOPROTEINS
MAN
GROWTH
SKELETAL DISEASES
ANIMALS
BODY
BODY AREAS
DISEASES
FACE
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MAMMALS
ORGANIC COMPOUNDS
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STRUCTURAL CHEMICAL ANALYSIS
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550400* - Genetics
550900 - Pathology