Molecular biology of coal bio-desulfurization; Quarterly technical progress report, January 1, 1991--March 31, 1991
The aim of this project is to use the techniques of molecular genetics to identify, clone, sequence, and enhance the expression of proteins which remove sulfur covalently bound to coal. This includes the movement and expression of these proteins into bacterial species which may be more useful in the industrial application of a biological desulfurization process. This quarter, several mutants were constructed to inactivate specific cloned C18 dox genes. These mutants were consistent with the phenotypes expected if these genes participated in an oxidative degradation DBT. The dox genes from strain A15 have been isolated in several cosmid clones, one of which can transfer the DBT metabolic trait to our laboratory Pseudomonas strain. DBT desulfurizing strains of Rhodococcus rhodochrous (IGTS8 and IGTS85) were obtained. Bioavailability assays confirmed the ability of these isolates to remove sulfur from DBT. Several mutants of IGTS8 were isolated that had lost the ability to use DBT as a sole sulfur source. These mutants were investigated as preferred recipients of the gene libraries. Multiple trials are underway to discover a mechanism by which DNA can be successfully introduced into the Rhodococcus strains. 1 tab.
- Research Organization:
- North Dakota Univ., Grand Forks, ND (United States). Dept. of Microbiology and Immunology
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC22-89PC89901
- OSTI ID:
- 721488
- Report Number(s):
- DOE/PC/89901-T4; ON: TI92020033
- Resource Relation:
- Other Information: PBD: 17 Apr 1991
- Country of Publication:
- United States
- Language:
- English
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