Molecular biology of coal bio-desulfurization; Quarterly technical progress report, October 1--December 31, 1990
The aim of this project is to use the techniques of molecular genetics to identify, clone, sequence, and enhance the expression of proteins which remove sulfur covalently bound to coal. This includes the movement and expression of these proteins into bacterial species which may be more useful in the industrial application of a biological desulfurization process. This quarter we finalized the initial cloning and sequencing of the dibenzothiophene (DBT) metabolic (``dox``) genes from strain C18. In addition, we constructed several mutations in single dox genes and have begun to dissect the contribution of each gene product in the DBT degradation pathway. Using a probe derived from DNA adjacent to a transposon which inactivated DBT metabolism, the DBT active genes from A15 have been cloned and identified on cosmids. We have also electroporated Thiobacillus ferrooxidans with a plasmid containing a chloramphenicol resistant transposon. Colonies of T. ferrooxidans resistant to chloramphenicol were obtained.
- Research Organization:
- North Dakota Univ., Grand Forks, ND (United States). Dept. of Chemistry
- Sponsoring Organization:
- USDOE, Washington, DC (USA)
- DOE Contract Number:
- AC22-89PC89901
- OSTI ID:
- 721276
- Report Number(s):
- DOE/PC/89901-T2; ON: TI91020991
- Resource Relation:
- Other Information: PBD: 25 Jan 1991
- Country of Publication:
- United States
- Language:
- English
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