Evaluation of the factors governing metal biosorption and metal toxicity in acidic soil isolates

Technical Report · Tue Jun 09 00:00:00 EDT 1992

OSTI ID:7206496

Pradhan, A A

This research project was designed to determine the feasibility of microbial biosorption processes for removing metal ions from aqueous systems. A culture of acidic soil actinomycetes, grown in an aerobic environment in a completely mixed, semibatch culture reactor, was used for the study. The experiments were based on removal of copper and lead from test solutions. The anionic systems tested were nitrate, sulfate, and chloride. To determine the factors influencing biosorption and to characterize metal uptake by cellular and extracellular components of the microbial system, a dialysis testing procedure was developed. The effectiveness of biosorption was influenced by pH, initial concentration of metals, type of anionic system, and organic content of the system. respirometric runs were carried out to identify potential inhibitory effects of metal accumulation on microbial activities. In general, metal accumulation resulted in a decrease in the microbial oxygen uptake rate. Also, a lag phase was observed before the onset of the respiratory activity particularly at concentrations of copper and lead greater than 100 ppM.

• Research Organization: Ames Lab., IA (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 7206496
• Report Number(s): IS-T-1523; ON: DE92016307
• Resource Relation: Other Information: Thesis (M.S.)
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
SOILS
CONTAMINATION
ACTINOMYCES
ANIONS
COMPILED DATA
COPPER
LEAD
MATHEMATICAL MODELS
METALS
PH VALUE
ROOT ABSORPTION
SOIL CHEMISTRY
TOXICITY
UPTAKE
ABSORPTION
BACTERIA
CHARGED PARTICLES
CHEMISTRY
DATA
ELEMENTS
INFORMATION
IONS
MICROORGANISMS
NUMERICAL DATA
SORPTION
TRANSITION ELEMENTS
540220* - Environment
Terrestrial- Chemicals Monitoring & Transport- (1990-)