A comparison of high performance gel permeation chromatography and nuclear magnetic resonance spectroscopy in the analysis of plasma from normal subjects and cancer patients

Journal Article · Fri Jan 01 00:00:00 EST 1988 · J. Liquid Chromatogr.; (United States)

DOI:https://doi.org/10.1080/01483918808068335· OSTI ID:7201761

Chmurny, G N; Mellini, M L; Halverson, D; Issaq, H J; Muschik, G M; Urba, W J; McGregor, G N; Hilton, B D; Caporaso, N; Smith, I C.P.

High Performance Gel Permeation Chromatography (GPC) was evaluated as an alternative to the more expensive Nuclear Magnetic Resonance (NMR) spectroscopy technique for cancer detection using human plasma. These two techniques show a biphasic relationship which can be explained on the basis of the relative amounts of the lipoprotein levels present in the plasma and a good correlation with total triglyceride concentrations obtained from standard blood tests. The major difference in the GPC elution profiles (254 nm) of plasma from normal individuals and that from cancer patients occurred in the peak eluting at the void volume. This peak has a retention time consistent with very low density lipoprotein (VLDL) and is elevated in most cancer patients and in normal patients with triglyceride levels greater than 200 mg/ml. The use of these techniques as a screening test for cancer in an asymptomatic population needs further evaluation.

• Research Organization: Programs Resources, Inc., NCI-FRCF, P.O. Box B, Frederick, MD (US)
• OSTI ID: 7201761
• Journal Information: J. Liquid Chromatogr.; (United States), Vol. 11:3
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
54 ENVIRONMENTAL SCIENCES
47 OTHER INSTRUMENTATION
BLOOD PLASMA
CHEMICAL ANALYSIS
GEL PERMEATION CHROMATOGRAPHY
LIPOPROTEINS
NMR SPECTRA
CARCINOMAS
BLOOD CHEMISTRY
BIOCHEMISTRY
COMPARATIVE EVALUATIONS
NMR SPECTROMETERS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHEMISTRY
CHROMATOGRAPHY
DISEASES
LIPIDS
MATERIALS
MEASURING INSTRUMENTS
NEOPLASMS
ORGANIC COMPOUNDS
PROTEINS
SEPARATION PROCESSES
SPECTRA
SPECTROMETERS
400102* - Chemical & Spectral Procedures
500200 - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
440300 - Miscellaneous Instruments- (-1989)