Analysis of 2,4,6-trinitrotoluene and its transformation products in soils and plant tissues by high-performance liquid chromatography
- Pacific Northwest Lab., Richland, WA (United States)
Previous studies have failed to provide an acceptable mass balance for 2,4,6-trinitrotoluene (TNT) in soils and plants due to deficiencies in analytical methodology. A high-performance liquid chromatographic (HPLC) method for soil analysis is reported which allowed for a mass balance in excess of 88% during a 2-month study. A method for plant analysis was developed which involved fractionation of organic extracts on Florisil adsorbent, to remove interfering pigments, followed by HPLC analysis of TNT and the primary metabolites, 2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene. Chromatographic recovery of TNT from spiked tissues was 85{plus minus}6%. The methodology was utilized to investigate TNT uptake and metabolism in plants grown in TNT hydroponic solutions.
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 7198183
- Journal Information:
- Journal of Chromatography (International Journal on Chromatography, Electrophoresis and Related Methods); (Netherlands), Vol. 518; ISSN 0021-9673
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
TNT
ENVIRONMENTAL TRANSPORT
QUANTITATIVE CHEMICAL ANALYSIS
DECOMPOSITION
EXPERIMENTAL DATA
LIQUID COLUMN CHROMATOGRAPHY
MASS BALANCE
METABOLITES
PLANT TISSUES
SOILS
UPTAKE
BODY
CHEMICAL ANALYSIS
CHEMICAL EXPLOSIVES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
EXPLOSIVES
INFORMATION
MASS TRANSFER
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SEPARATION PROCESSES
TISSUES
540210* - Environment
Terrestrial- Basic Studies- (1990-)
400105 - Separation Procedures