Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Journal of Bacteriology; (USA)

OSTI ID:7195193

Machlin, S M; Hanson, R S ^[1]

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1. Univ. of Minnesota, Navarre (USA)

The nucleotide sequence of a cloned 2.5-kilobase-pair SmaI fragment containing the methanol dehydrogenase (MDH) structural gene from Methylobacterium organophilum XX was determined. A single open reading frame with a coding capacity of 626 amino acids (molecular weight, 66,000) was identified on one stand, and N-terminal sequencing of purified MDH revealed that 27 of these residues constituted a putative signal peptide. Primer extension mapping of in vivo transcripts indicated that the start of mRNA synthesis was 160 to 170 base pairs upstream of the ATG codon. Northern (RNA) blot analysis further demonstrated that the transcript was 2.1 kilobase pairs in length and therefore appeared to encode only MDH.

• DOE Contract Number: AC02-82ER12029
• OSTI ID: 7195193
• Journal Information: Journal of Bacteriology; (USA), Vol. 170:10; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALCOHOL DEHYDROGENASE
DNA SEQUENCING
BACTERIA
MOLECULAR BIOLOGY
GENETIC MAPPING
METHANOL
TRANSCRIPTION
ALCOHOLS
ENZYMES
HEMIACETAL DEHYDROGENASES
HYDROXY COMPOUNDS
MAPPING
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
550400 - Genetics