Selective expression of a protein-tyrosine kinase, p56 sup lyn , in hematopoietic cells and association with production of human T-cell lymphotropic virus type I

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6538· OSTI ID:7195149

Yamanashi, Yuji; Mori, Shigeo; Inoue, Kazushi; Yamamoto, Tadashi; Toyoshima, Kumao ^[1]; Yoshida, Mitsuaki ^[2]; Kishimoto, Tadamitsu ^[3]

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1. Univ. of Tokyo (Japan)
2. Cancer Institute, Tokyo (Japan)
3. Osaka Univ. (Japan)

This paper reports the identification of the lyn gene product, a member of the src-related family of protein-tyrosine kinases, and its expression in hematopoietic cells. A lyn-specific sequence (Arg-25 to Ala-119 of the protein) was expressed in Escherichia coli as a fusion protein with {beta}-galactosidase. Antiserum raised against the fusion protein immunoprecipitated a 56-kDa protein from human B lymphocytes. Incubation of the immunoprecipitate with ({gamma}-{sup 32}P)ATP resulted in the phosphorylation of this protein at tyrosine residues. Immunohistological and immunoblotting analyses showed that the lyn gene product was expressed in lymphatic tissues (spleen and tonsil) and in adult lung, which contains many macrophages. Furthermore, both the transcripts and the protein products of the lyn gene accumulated in macrophages/monocytes, platelets, and B lymphocytes but were not expressed appreciably in granulocytes, erythrocytes, or T lymphocytes, suggesting that lyn gene products function primarily in certain differentiated cells of lymphoid and myeloid lineages.

• OSTI ID: 7195149
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
CYTOCHEMISTRY
PHOSPHOTRANSFERASES
SUBCELLULAR DISTRIBUTION
TYROSINE
PHOSPHORYLATION
VIRUSES
CHEMICAL ACTIVATION
AMINO ACID SEQUENCE
ATP
ESCHERICHIA COLI
GENES
LUNGS
LYMPHOCYTES
MACROPHAGES
MAN
MONOCLONAL ANTIBODIES
PHOSPHORUS 32
RECOMBINANT DNA
SPLEEN
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DNA
ENZYMES
HYDROXY ACIDS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHAGOCYTES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
RADIOISOTOPES
RESPIRATORY SYSTEM
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques