Effects of chronic exposure to ozone on collagen in rat lung

Journal Article · Tue Mar 15 00:00:00 EST 1988 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(88)90184-6· OSTI ID:7194808

Wright, E S; Kehrer, J P; White, D M; Smiler, K L

Pulmonary fibrosis is a consequence of severe injury from some toxic agents including high doses of ozone. It is not known, however, whether chronic exposure to low doses of ozone, such as those encountered in polluted ambient atmospheres, could also result in abnormal accumulations of lung collagen. Rats were exposed to ozone for 20 hr per day, 7 days per week for 3, 6, 12, and 18 months at concentrations of 0.12, 0.25, or 0.50 ppm. Controls were exposed under identical conditions to purified air. Upon removal from the chambers, rats were euthanized and lung tissue slices incubated with (14C)proline. The incorporation of 14C into hydroxyproline and the total hydroxyproline content of lung tissue were measured as estimates of lung collagen synthesis and content, respectively. The formation of labeled hydroxyproline tended to decrease significantly with time in controls and at the three ozone doses. There were, however, no significant dose-related changes at any of the time points tested. Total lung hydroxyproline increased with age in all groups, but no dose-related changes were detected at any time point. It was concluded that chronic exposure of rats to ozone at concentrations which approximate ambient urban concentrations did not affect normal age-related changes in either synthesis or accumulation of lung collagen.

• Research Organization: General Motors Research Labs, Warren, MI (USA)
• OSTI ID: 7194808
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Vol. 92:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COLLAGEN
BIOSYNTHESIS
LUNGS
FIBROSIS
OZONE
TOXICITY
AIR POLLUTION
CARBON 14 COMPOUNDS
CHRONIC EXPOSURE
DOSE-RESPONSE RELATIONSHIPS
HYDROXYPROLINE
RATS
TRACER TECHNIQUES
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BODY
CARBOXYLIC ACIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
POLLUTION
PROTEINS
PYRROLES
PYRROLIDINES
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology