Quantitative determination of the intracellular fate of internalized plasma membrane in dissociated pituitary prolactin cells utilizing a radioiodinated cationic ferritin probe (CFI) and electron microscopic autoradiography

Journal Article · Wed Feb 01 00:00:00 EST 1984 · Am. J. Anat.; (United States)

DOI:https://doi.org/10.1002/aja.1001690207· OSTI ID:7192190

Rosenzweig, L J; Kanwar, Y S

Dissociated anterior pituitary cells derived from estrogen-treated female rats were incubated with radioiodinated cationic ferritin (CFI) for 2 min and subsequently in the absence of CFI for varying periods of time up to 3 hr in order to quantitate, using electron microscopic autoradiography, the distribution of retrieved plasma membrane in these cells. Following a 2-min incubation with CFI, autoradiographic grains were found to be associated almost exclusively with the plasma membrane. With increasing periods of incubation in the absence of CFI, grain-density analysis revealed increasing levels of CFI in multiple intracellular organelles. The levels of CFI were greatest for the lysosomes, intermediate for the mature secretory granules, and least for the Golgi cisternae and immature secretory granules. These findings are consistent with the idea that a portion of the retrieved plasma membrane is degraded in lysosomes and that the remainder is recycled to organelles comprising the secretory pathway to be reutilized in successive waves of the secretory cycle.

• Research Organization: Department of Veterinary Biology, College of Veterinary Medicine, University of Minnesota, St. Paul
• OSTI ID: 7192190
• Journal Information: Am. J. Anat.; (United States), Vol. 169:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PITUITARY GLAND
AUTORADIOGRAPHY
CELL MEMBRANES
ELECTRON MICROSCOPY
FERRITIN
IODINE ISOTOPES
LTH
LYSOSOMES
RATS
ANIMALS
BODY
CELL CONSTITUENTS
COMPLEXES
ENDOCRINE GLANDS
GLANDS
GONADOTROPINS
HORMONES
IRON COMPLEXES
ISOTOPES
MAMMALS
MEMBRANES
METALLOPROTEINS
MICROSCOPY
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RODENTS
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
550301* - Cytology- Tracer Techniques