Biochemical and subcellular distribution of arachidonic acid in rat myocardium

Journal Article · Tue Dec 01 00:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7191815

Miyazaki, Y; Gross, R W; Sobel, B E; Saffitz, J E ^[1]

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1. Washington Univ., St. Louis, MO (USA)

Selective release of arachidonic acid from prelabeled phospholipid pools has been observed following exposure of neonatal rat cardiac myocytes to metabolic inhibitors in vitro and has been correlated temporally with the development of irreversible sarcolemmal damage. Hydrolysis of phospholipids with release of arachidonic acid may be an important mechanism in the pathogenesis of sarcolemmal damage induced by ischemia. To elucidate potential subcellular loci of arachidonic acid release in ischemic myocardium, the authors characterized the phospholipid composition of adult rat myocardial sarcolemma and delineated the biochemical and subcellular distribution of radiolabeled arachidonic acid in neonatal rat myocytes incubated with ({sup 3}H)-arachidonic acid for selected intervals. Radioactivity was located almost exclusively in mitochondria and internal cytoplasmic membranes (primarily sarcoplasmic reticulum), which collectively contained 90% of myocyte radioactivity. These results indicate that radiolabeled arachidonic acid released from prelabeled phospholipid pools on exposure of neonatal rat myocytes to oxidative inhibitors is derived from mitochondria and internal cell membranes. The diminutive labeling of the sarcolemma suggests that turnover of arachidonoyl phospholipids is slower in the sarcolemma than in other membranous organelles.

• OSTI ID: 7191815
• Journal Information: American Journal of Physiology; (USA), Vol. 253:6; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
SUBCELLULAR DISTRIBUTION
MYOCARDIUM
AUTORADIOGRAPHY
PHOSPHOLIPIDS
HYDROLYSIS
TRITIUM COMPOUNDS
CELL CONSTITUENTS
CELL CULTURES
HEART
ISCHEMIA
RATS
TRANSMISSION ELECTRON MICROSCOPY
ANIMALS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
DECOMPOSITION
DISEASES
DISTRIBUTION
ELECTRON MICROSCOPY
ESTERS
HYDROGEN COMPOUNDS
LIPIDS
LYSIS
MAMMALS
MICROSCOPY
MONOCARBOXYLIC ACIDS
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
RODENTS
SOLVOLYSIS
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques