(/sup 3/H)TCP as a potential anatomical marker for N-methyl-D-aspartate receptors
Quantitative autoradiography was used to determine whether N-methyl-D-aspartate (NMDA) binding sites and dissociative anesthetic (DSA) sites were anatomically associated in rat brain tissue. Binding to the NMDA receptor was accomplished by incubating the brain sections with (/sup 3/H)-glutamate under NMDA preferring conditions. The high affinity phencyclidine (PCP) derivative (/sup 3/H) N-(1-(2-thienyl) cyclohexy 1)3,4-piperidine (TCP) was used to label the DSA sites. In the normal rat brain the relative binding pattern of (/sup 3/H)TCP was virtually identical to that of NMDA receptor distribution. Only in the granule cell layer of the cerebellum was there a lack of concordance between the two receptors. The stoichiometry of binding indicated that there are 4-5 NMDA sites for every one dissociative anesthetic binding site. In order to determine whether NMDA and DSA receptors existed on pre- or postsynaptic elements, autoradiography of both sites was carried out in rats which had previously received selective lesions of either cortical or hippocampal afferent pathways or lesions which destroyed only the intrinsic neurons in these structures.
- Research Organization:
- Michigan Univ., Ann Arbor (USA)
- OSTI ID:
- 7191683
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques