Biosynthesis of the tonoplast H sup + -ATPase

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:7191647

Randall, S K ^[1]; Sze, H ^[2]

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1. McGill Univ., Montreal, Quebec (Canada)
2. Univ. of Maryland, College Park (USA)

To determine whether the tonoplast H{sup +}-ATPase was differentially synthesized in oat seedlings, sections were labeled in vivo with ({sup 35}S)-methionine and ATPase subunits were immunoprecipitated. Subunits were detected in all portions of the seedling with the exception of the seed. The intracellular site of synthesis for two peripheral ATPase subunits was investigated. RNA encoding the 72 kDa (catalytic) subunit was found in membrane-bound polysomes. In contrast, message for the 60 kDa subunit was found on free polysomes. Polypeptides synthesized in vivo or obtained from RNA translated in vitro exhibited no apparent size differences, suggesting the absence of cleaved precursors for the 72 or 60 kDa subunits.

• OSTI ID: 7191647
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP-ASE
BIOCHEMICAL REACTION KINETICS
GENE REGULATION
IN VIVO
LABELLED COMPOUNDS
LABELLING
METHIONINE
OATS
RNA
SEEDLINGS
SULFUR 35
TRACER TECHNIQUES
ACID ANHYDRASES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CEREALS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GRASS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LILIOPSIDA
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOHYDROLASES
PLANTS
RADIOISOTOPES
REACTION KINETICS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques