Toward a physical map of the genome of the nematode Caenorhabditis elegans

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.20.7821· OSTI ID:7191488

Coulson, A; Sulston, J; Brenner, S; Karn, J

A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.

• Research Organization: Medical Research Council Library of Molecular Biology, Cambridge (England)
• OSTI ID: 7191488
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NEMATODES
DNA SEQUENCING
GENETIC MAPPING
ATP
COMPUTER CODES
DNA
ELECTROPHORESIS
GENOTYPE
PHOSPHORUS 32
SULFUR 35
ASCHELMINTHES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HELMINTHS
ISOTOPES
LIGHT NUCLEI
MAPPING
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
550401* - Genetics- Tracer Techniques
550201 - Biochemistry- Tracer Techniques