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Title: Measurement of non-steady-state free fatty acid turnover

Abstract

The accuracy of non-steady-state equations for measuring changes in free fatty acid rate of appearance (Ra) is unknown. In the present study, endogenous lipolysis (traced with ({sup 14}C)-linoleate) was pharmacologically suppressed in six conscious mongrel dogs. A computer-responsive infusion pump was then used to deliver an intravenous oleic acid emulsion in both constant and linear gradient infusion modes. Both non-steady-state equations with various effective volumes of distribution (V) and steady-state equations were used to measure oleate Ra (({sup 14}C)oleate). Endogenous lipolysis did not change during the experiment. When oleate Ra increased in a linear gradient fashion, only non-steady-state equations with a large (150 ml/kg) V resulted in erroneous values (9% overestimate, P less than 0.05). In contrast, when oleate Ra decreased in a similar fashion, steady-state and standard non-steady-state equations (V = plasma volume = 50 ml/kg) overestimated total oleate Ra (18 and 7%, P less than 0.001 and P less than 0.05, respectively). Overall, non-steady-state equations with an effective V of 90 ml/kg (1.8 x plasma volume) allowed the most accurate estimates of oleate Ra.

Authors:
; ;  [1]
  1. Mayo Clinic, Rochester, MN (USA)
Publication Date:
OSTI Identifier:
7191481
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 258; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LINOLEIC ACID; METABOLISM; OLEIC ACID; BIOLOGICAL MODELS; CARBON 14 COMPOUNDS; DOGS; EQUATIONS; TRACER TECHNIQUES; ANIMALS; CARBOXYLIC ACIDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Jensen, M D, Heiling, V, and Miles, J M. Measurement of non-steady-state free fatty acid turnover. United States: N. p., 1990. Web.
Jensen, M D, Heiling, V, & Miles, J M. Measurement of non-steady-state free fatty acid turnover. United States.
Jensen, M D, Heiling, V, and Miles, J M. 1990. "Measurement of non-steady-state free fatty acid turnover". United States.
@article{osti_7191481,
title = {Measurement of non-steady-state free fatty acid turnover},
author = {Jensen, M D and Heiling, V and Miles, J M},
abstractNote = {The accuracy of non-steady-state equations for measuring changes in free fatty acid rate of appearance (Ra) is unknown. In the present study, endogenous lipolysis (traced with ({sup 14}C)-linoleate) was pharmacologically suppressed in six conscious mongrel dogs. A computer-responsive infusion pump was then used to deliver an intravenous oleic acid emulsion in both constant and linear gradient infusion modes. Both non-steady-state equations with various effective volumes of distribution (V) and steady-state equations were used to measure oleate Ra (({sup 14}C)oleate). Endogenous lipolysis did not change during the experiment. When oleate Ra increased in a linear gradient fashion, only non-steady-state equations with a large (150 ml/kg) V resulted in erroneous values (9% overestimate, P less than 0.05). In contrast, when oleate Ra decreased in a similar fashion, steady-state and standard non-steady-state equations (V = plasma volume = 50 ml/kg) overestimated total oleate Ra (18 and 7%, P less than 0.001 and P less than 0.05, respectively). Overall, non-steady-state equations with an effective V of 90 ml/kg (1.8 x plasma volume) allowed the most accurate estimates of oleate Ra.},
doi = {},
url = {https://www.osti.gov/biblio/7191481}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 258,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 1990},
month = {Mon Jan 01 00:00:00 EST 1990}
}