Heterogeneity in the properties of burst-forming units of erythroid lineage in sickle cell anemia: DNA synthesis and burst-promoting activity production is related to peripheral hemoglobin F levels

Journal Article · Thu Feb 15 00:00:00 EST 1990 · Blood (Journal of Hematology); (USA)

OSTI ID:7190549

Croizat, H; Billett, H H; Nagel, R L ^[1]

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1. Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY (USA)

Circulating 14-day erythroid progenitors (BFU-E) from 28 sickle cell anemia (SS) patients with hemoglobin F (HbF) levels ranging from 2% to 16% were studied to determine their sensitivity to ({sup 3}H) thymidine kill and burst-promoting activity (BPA)-like factor production. We find that the proportion of BFU-E sensitive to 3H-dT kill, and hence active in DNA synthesis, was inversely correlated with the percent of peripheral HbF when light density (LD) mononuclear cells were used for plating. Regression analysis showed that the correlation between HbF level and BFU-E kill was highly significant (r = .88; P less than .00003). We confirmed the BPA-like factor(s) production by LD mononuclear cells of SS patients, and found, in addition, that this phenomenon is restricted to the population of SS patients with HbF levels lower than 9%. Circulating BFU-E of patients with high HbF levels are not sensitive to 3H-dT, and their mononuclear cells do not release BPA-like factor. In summary, SS patients exhibit differences in the capacity of their mononuclear cells to produce BPA activity according to their peripheral HbF level, as well as to the DNA synthesis-state of their circulating BFU-E. We conclude that erythroid progenitors differ among SS patients in relation to their peripheral HbF level.

• OSTI ID: 7190549
• Journal Information: Blood (Journal of Hematology); (USA), Vol. 75:4; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEMOGLOBIN
BIOSYNTHESIS
SICKLE CELL ANEMIA
PATHOGENESIS
CELL DIVISION
CELL KILLING
DNA
DNA REPLICATION
MAN
MONOCYTES
PATIENTS
REGRESSION ANALYSIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANEMIAS
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DISEASES
GLOBINS
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEUKOCYTES
MAMMALS
MATERIALS
MATHEMATICS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
STATISTICS
SYMPTOMS
SYNTHESIS
VERTEBRATES
550901* - Pathology- Tracer Techniques