Activation and regulation of arachidonic acid release in rabbit peritoneal neutrophils
Arachidonic acid release in rabbit neutrophils can be enhanced by the addition of chemotactic fMet-Leu-Phe, platelet-activating factor, PAF, or the calcium ionophore A23187. Over 80% of the release ({sup 3}H)arachidonic acid comes from phosphatidylcholine and phosphatidylinositol. The release is dose-dependent and increases with increasing concentration of the stimulus. The A23187-induced release increases with increasing time of the stimulation. ({sup 3}H)arachidonic acid release, but not the rise in the concentration of intracellular calcium, is inhibited in pertussis toxin-treated neutrophils stimulated with PAF. The ({sup 3}H)arachidonic acid released by A23187 is potentiated while that release by fMET-Leu-Phe or PAF is inhibited in phorbol 12-myristate 13-acetate, PMA, treated rabbit neutrophils. The protein kinase C inhibitor 1-(5-isoquinoline sulfonyl)-2-methylpiperazine, H-7, has no effect on the potentiation by PMA of the A23187-induced release, it prevents the inhibition by PMA of the release produced by PAF or fMet-Leu-Phe. In addition, PMA increases arachidonic acid release in H-7-treated cells stimulated with fMet-Leu-Phe. The diacylglycerol kinase inhibitor R59022 increases the level of diacylglycerol in neutrophils stimulated with fMet-Leu-Phe. Furthermore, R59022 potentiates ({sup 3}H) arachidonic acid release produced by fMet-Leu-Phe. This potentiation is not inhibited by H-7, in fact, it is increased in H-7-treated neutrophils.
- Research Organization:
- Connecticut Univ., Storrs, CT (USA)
- OSTI ID:
- 7189609
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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ARACHIDONIC ACID
SECRETION
BLOOD COAGULATION FACTORS
BIOLOGICAL FUNCTIONS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
NEUTROPHILS
PHOSPHOLIPIDS
RABBITS
TRACER TECHNIQUES
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ANIMALS
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BLOOD
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BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
COAGULANTS
DRUGS
ESTERS
FUNCTIONS
HEMATOLOGIC AGENTS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEUKOCYTES
LIPIDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PROTEINS
VERTEBRATES
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