Stimulation of the synthesis of 15-hydroxyeicosatetraenoic acid (15-HETE) and 6-keto-prostaglandin F/sub 1. cap alpha. / (6-keto-PGF/sub 1. cap alpha. /) by cultured human umbilical veins
These studies were designed to investigate the synthesis of 6-keto-PGF/sub 1..cap alpha../ and 15-HETE in cultured human endothelial cells. The identification of the 15-HETE in these cells was made by UV absorption and gas chromatography/mass spectrometry. Specific radioimmunoassays were developed to quantify the synthesized 6-keto-PGF/sub 1..cap alpha../ and 15-HETE. The release of 15-HETE and 6-keto-PGF/sub 1..cap alpha../ was stimulated by arachidonic acid, histamine or the calcium ionophore A23187. The release of 15-HETE paralleled the release of 6-keto-PGF/sub 1..cap alpha../ and was both concentration-related and time-dependent. Aspirin, ibuprofen and indomethacin inhibited both the formation of 6-keto-PGF/sub 1..cap alpha../ and 15-HETE in similar concentrations. These data indicate that agents which stimulate PGI/sub 2/ synthesis also stimulate the synthesis of 15-HETE. Also, they implicate the cyclooxygenase pathway in the synthesis of 6-keto PGF/sub 1..cap alpha../ and 15-HETE in human endothelial cells.
- Research Organization:
- Univ. of Texas Health Science Center, Dallas
- OSTI ID:
- 7189314
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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ENDOTHELIUM
HISTAMINE
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550201* - Biochemistry- Tracer Techniques