Interaction of osteoblast-like cells with serum and fibronectin: effects on cell motility and proliferation in vitro
Osteoblast migration and proliferation are believed to occur during bone remodelling, in particular after osteoclastic bone resorption and prior to osteoblastic bone formation. In order to study migration and proliferation in vitro, the model of Alessandri et al. (1983) was modified. The model entailed seeding osteoblast-like cells into wells cut in agar and quantifying migration and proliferation peripheral to the well. Cell morphology also was described. The data indicated that on growth surfaces enriched with varying concentrations of fetal calf serum (FSC), the quantification of migration and proliferation was related both to percent cell attachment and to FCS-concentration. Because few osteoblast-like cells incorporated (/sup 3/H-TdR), it was concluded that the appearance of cells peripheral to the well was due to migration, and not to proliferation. Cell morphology and myosin distribution and organization indicated that osteoblast-like cells at the periphery of the cell culture (i.e. leading edge) may have been directionally migrating whereas cells behind the leading edge may have been engaged in non-directional migration. The migration, proliferation, and morphology of osteoblast-like cells cultured on fibronectin (FN) enriched growth surfaces also was examined. The quantification of migration and proliferation was related to the FN-concentration applied to the growth surface. Because few osteoblast-like cells incorporated /sup 3/H-TdR and cell morphology indicated migration, it was concluded that osteoblast-like cells on FN-enriched growth surfaces are specialized, in part, for migration.
- Research Organization:
- Loyola Univ., Chicago, IL (USA)
- OSTI ID:
- 7189123
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Collagen VI suppresses fibronectin-induced enteric neural crest cell migration by downregulation of focal adhesion proteins
Isolation and characterization of human islet stellate cells
Related Subjects
BONE CELLS
CELL PROLIFERATION
MORPHOLOGICAL CHANGES
FIBRINOLYTIC AGENTS
BIOLOGICAL EFFECTS
TRITIUM COMPOUNDS
UPTAKE
BLOOD SERUM
IN VITRO
THYMIDINE
ANIMAL CELLS
AZINES
CONNECTIVE TISSUE CELLS
DRUGS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
550301* - Cytology- Tracer Techniques