New primate model for the study of intravenous thrombotic potential and its modification
Advances in venous reconstruction have been limited by inherent venous thrombogenicity and the absence of a suitable prosthetic material for use in the venous system. We have designed an in vivo experimental model to evaluate early blood-material interactions within the venous system and to quantitate drug efficacy in the alteration of platelet function and fibrin deposition in the baboon. An 8F catheter was placed percutaneously in the femoral vein of an adult male baboon. Indium 111-labeled autogenous platelets or iodine 125-labeled human fibrinogen was infused before the introduction, into the inferior vena cava, of a linear array of 5 x 15 mm alternating Dacron and polytetrafluoroethylene samples attached to a benzalkonium-heparin-treated guide wire. At 60 or 120 minutes the samples were removed and a 1 ml aliquot of blood was drawn. The materials and blood samples were counted in a gamma well counter, and the material counts were normalized to the circulating label present in the 1 ml blood sample. The experiment was repeated after pretreatment with heparin, aspirin, or dextran. Whole blood clotting times and bleeding times were monitored. The results showed decreased platelet and fibrin deposition on polytetrafluoroethylene when compared with Dacron in the venous system. Aspirin, heparin, and dextran were all found to decrease platelet and fibrin deposition onto intravenously placed graft material samples (p less than 0.05, Student's t test). The data confirm the ability of the model to evaluate quantitatively anticoagulants, antiplatelet agents, and prospective graft materials for use in venous reconstructions.
- Research Organization:
- Tufts Univ. School of Medicine, Boston, MA (USA)
- OSTI ID:
- 7185482
- Journal Information:
- J. Vasc. Surg.; (United States), Vol. 8:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
THROMBOSIS
BIOLOGICAL MODELS
VEINS
GRAFTS
ANTICOAGULANTS
APES
BLOOD COAGULATION
BLOOD PLATELETS
FIBRINOGEN
HEPARIN
INDIUM 111
IODINE 125
MATERIALS TESTING
POLYTETRAFLUOROETHYLENE
TRACER TECHNIQUES
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
COAGULANTS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FLUORINATED ALIPHATIC HYDROCARBONS
GLOBULINS
HALOGENATED ALIPHATIC HYDROCARBONS
HEMATOLOGIC AGENTS
HEMOSTATICS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC POLYMERS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYETHYLENES
POLYMERS
POLYOLEFINS
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
TESTING
TRANSPLANTS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques