Studies on the structure and biosynthesis of the linkage region between chitin and protein in Artemia salina
In vivo and in vitro studies on chitin synthesis in Artemia nauplii indicate that two classes of /sup 3/H GlcNAc labeled products are synthesized. The first is soluble in urea and contains a chitoprotein with short chitin oligosaccharides and attached to a high molecular weight polypeptide. The other product is insoluble in urea and SDS, and contains crosslinked, macromolecular chitin. Both classes of chitin products bind the fluorescent probe Calcofluor White (CW), as do glycopeptides prepared from these samples by pronase digestion. The in vivo and in vitro effect of CW on synthesis of both chitin products has been examined. /sup 3/H GlcNAc-labeled glycopeptides have been generated from both products by HCl partial hydrolysis, CNBr cleavage or pronase digestion. Analysis of the pronase soluble fraction after digestion of the urea residue has been carried out by gel permeation and paper chromatography. Sic glycopeptide fractions have been isolated which contain up to 85% (w/w) GlcN and 4-6 predominant amino acids. Common amino acids in all fractions are Asp, Arg and Thr; others found include Lys, Glu and Ala. Synthesis of /sup 3/H GlcNAc-labeled chitopeptides has been achieved in vitro using artificial peptide acceptors such as DNS-Ala-Ile-Glu-Asn-Ala-Thr-Leu and N/sup ..cap alpha../-/sup 3/H Ac-Asn-Tyr-Thr-NHCH/sub 3/. Further studies on the synthesis of Dol-PP-(GlcNAc)/sub 3-8/ and the properties of the oligosaccharide transferase are in progress.
- Research Organization:
- Mercer Univ., Macon, GA
- OSTI ID:
- 7171186
- Report Number(s):
- CONF-8606151-; TRN: 86-039078
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHITIN
BIOSYNTHESIS
PROTEIN STRUCTURE
GLUCOPROTEINS
SHRIMP
BIOCHEMISTRY
CHROMATOGRAPHY
IN VITRO
IN VIVO
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
CARBOHYDRATES
CHEMISTRY
CRUSTACEANS
DECAPODS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques