UVB-induced epidermal hyperproliferation is modified by a single, topical treatment with a mitosis inhibitory epidermal pentapeptide
- Univ. of Oslo, Rikshospitalet (Norway)
A single application of a water-miscible cream base containing the recently identified mitosis inhibitory epidermal pentapeptide pyroGlu-Glu-Asp-Ser-GlyOH (EPP) to hairless mouse skin is followed by a long-lasting period of reduced epidermal cell proliferation. To examine if a similar growth inhibition could be achieved in stimulated and rapidly proliferating epidermis, EPP was applied at two different concentrations, 0.005 or 0.02%, to hairless mouse skin immediately after exposure of the left flank to an erythemic dose of ultraviolet B light (UVB). This dose of UVB alone induces a sustained period of rapid epidermal cell proliferation, starting at about 18 h after the irradiation. Epidermal cell proliferation was followed from 18 to 54 h (0.005% cream) or from 18 to 30 h (0.02% cream) after the treatment by estimating the rate of G2-M cell flux (the mitotic rate) by means of Colcemid, and epidermal DNA synthesis by counting labeled cells after pulse-labeling with 3H-thymidine. The unirradiated side of the mice was used as reference. The results showed that topical treatment with a 0.02% EPP cream partially inhibited UVB-induced epidermal hyperproliferation, while the 0.005% EPP cream inhibited as well as stimulated the UVB-induced hyperproliferation. Thus, EPP is effective even in rapidly proliferating epidermal cell populations, but the outcome is obviously dose-dependent in this test system.
- OSTI ID:
- 7167766
- Journal Information:
- Journal of Investigative Dermatology; (USA), Vol. 94:1; ISSN 0022-202X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
CELL PROLIFERATION
PEPTIDES
RADIOSENSITIVITY EFFECTS
SKIN
BIOLOGICAL RADIATION EFFECTS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
EPIDERMIS
INHIBITION
MICE
MITOSIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ANIMAL TISSUES
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BODY
CELL DIVISION
ELECTROMAGNETIC RADIATION
EPITHELIUM
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
RODENTS
TISSUES
VERTEBRATES
550901* - Pathology- Tracer Techniques