Effect of gamma radiation on sodium channels in different conformations in neuroblastoma cells

Technical Report · Wed Jan 01 00:00:00 EST 1986

OSTI ID:7164634

Freschi, J E; Moran,

The dose-response relationship between gamma radiation and batrachotoxin-stimulated sodium influx in neuroblastoma cells in tissue culture was studied. Also tested was the hypothesis that changes in sodium-channel conformation may alter the radiosensitivity of the channel. It was found that gamma radiation inhibited toxin-stimulated /sup 22/Na uptake at doses beyond a threshold of 200-300 Gy. No effects were seen following doses below 100 Gy. This inhibition of sodium permeability was seen when the cells were irradiated with sodium channels in the closed or inactivated, nonconducting states. However, when the channels were in the toxin-opened, conducting state, gamma radiation had no effect at doses up to 2000 Gy. Results support earlier electrophysiological studies that showed that high doses of ionizing radiation are required to produce a measureable decrease in sodium permeability. In addition, the data suggest that by changing the sodium-channel conformation, batrachotoxin appears to alter radiosensitive chemical bonds in the gating or ion-conducting portion of the channel.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
• OSTI ID: 7164634
• Report Number(s): AD-A-172795/7/XAB; AFRRI-SR-86-26
• Country of Publication: United States
• Language: English

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