Production of leukotrienes by macrophage cells irradiated with ultraviolet light
Mouse peritoneal macrophages were cultured, labelled with /sup 14/C-arachidonic acid, and then were irradiated with UV light (254 nm). Also, some /sup 14/C-arachidonic acid labelled macrophages were treated with Ca-ionophore (A-23187). The UV-treated macrophages produced two to three times as much arachidonic acid metabolites as did the Ca-ionophore treated cells, the UV irradiated cells produced about 20 ng of LTC/sub 4/ and 5 ng of LTB/sub 4/ per million cells, whereas the Ca-ionophore treated cells produced 10 ng LTC/sub 4/ and 1 ng LTB/sub 4/ per million cells. The irradiated cultures also exhibited a high degree of aggregation of viable macrophages around the lysed cells. There was little aggregation in the Ca-ionophore treated cultures. In phagocytosis and cell aggregation leukotrienes are produced by the viable macrophage cells. Leukotrienes are arachidonic acid oxygenation products that are thought to be mediators both in the expression of the immune-based and inflammatory responses. This study shows that macrophage cells under stressful conditions produced by a trauma-causing agent (UV light) respond by producing leukotrienes and chemotactic factors. These responses of the macrophage cells are the result of multiple biochemical events that promote the production of leukotrienes in the cultures.
- Research Organization:
- Texas Woman's Univ., Denton (USA)
- OSTI ID:
- 7161685
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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ARACHIDONIC ACID
METABOLITES
CARBON 14 COMPOUNDS
MACROPHAGES
BIOLOGICAL RADIATION EFFECTS
AGGLOMERATION
EXTREME ULTRAVIOLET RADIATION
MICE
OXIDATION
PHAGOCYTOSIS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture