Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi)

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Genetics; (United States)

OSTI ID:7160896

Klittich, C J.R.; Leslie, J F

Twelve strains of Fusarium moniliforme were examined for their ability to sector spontaneously on toxic chlorate medium. All strains sectored frequently; 91% of over 1200 colonies examined formed chlorate-resistant, mutant sectors. Most of these mutants had lesions in the nitrate reduction pathway and were unable to utilize nitrate (nit mutants). nit mutations occurred in seven loci: a structural gene for nitrate reductase (nit1), a regulatory gene specific for the nitrate reduction pathway (nit3), and five genes controlling the production of a molybdenum-containing cofactors that is necessary for nitrate reductase activity (nit2, nit4, nit5, nit6, nit7). No mutations affecting nitrite reductase or a major nitrogen regulatory locus were found among over 1000 nit mutants. Mutations of nit1 were recovered most frequently (39-66%, depending on the strain) followed by nit3 mutations (23-42%). The frequency of isolation of each mutant type could be altered, however, by changing the source of nitrogen in the chlorate medium. The authors concluded that genetic control of nitrate reduction in F. moniliforme is similar to that in Aspergillus and Neurospora, but that the overall regulation of nitrogen metabolism may be different.

• Research Organization: Kansas State Univ., Manhattan (USA)
• OSTI ID: 7160896
• Journal Information: Genetics; (United States), Vol. 118:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
NITRATES
BIOLOGICAL PATHWAYS
GENE REGULATION
REDUCTION
FUSARIUM
GENES
GENETIC MAPPING
METABOLISM
MUTANTS
CHEMICAL REACTIONS
FUNGI
MAPPING
NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PLANTS
560300* - Chemicals Metabolism & Toxicology
550400 - Genetics