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Title: Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21

Abstract

A gene designated BRCA1, implicated in the susceptibility to early-onset familial breast cancer, has recently been localized to chromosome 17q12-q21. To date, the order of DNA markers mapped within this region has been based on genetic linkage analysis. The authors report the use of multicolor fluorescence in situ hybridization to establish a physically based map of five polymorphic DNA markers and 10 cloned genes spanning this region. Three cosmid clones and Alu-PCR-Generated products derived from 12 yeast artificial chromosome clones representing each of these markers were used in two-color mapping experiments to determine an initial proximity of markers relative to each other on metaphase chromosomes. Interphase mapping was then employed to determine the order and orientation of closely spaced loci by direct visualization of fluorescent signals following hybridization of three probes, each detected in a different color. Statistical analysis of the combined data suggests that the order of markers in the BRCA1 regions is cen-THRA1-TOP2-GAS-OF2-17HSD-248yg9-RNU2-OF3-PPY/p131-EPB3-Mfd188-WNT3-HOX2-GP3A-tel. This map is consistent with that determined by radiation-reduced hybrid mapping and will facilitate positional cloning strategies in efforts to isolate and characterize the BRCA1 gene. 27 refs., 2 figs., 3 tabs.

Authors:
; ; ; ; ; ;  [1];  [2];  [3];  [4]
  1. Univ. of Michigan Human Genome Center, Ann Arbor, MI (United States) Howard Hughes Medical Institute, Ann Arbor, MI (United States)
  2. Univ. of California, Berkeley, CA (United States)
  3. Univ. of Michigan Human Genome Center, Ann Arbor, MI (United States)
  4. Howard Hughes Medical Institute, Ann Arbor, MI (United States)
Publication Date:
OSTI Identifier:
7160547
Resource Type:
Journal Article
Journal Name:
Genomics; (United States)
Additional Journal Information:
Journal Volume: 17:3; Journal ID: ISSN 0888-7543
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 17; GENETIC MAPPING; MAMMARY GLANDS; HEREDITARY DISEASES; NEOPLASMS; DNA HYBRIDIZATION; BODY; CHROMOSOMES; DISEASES; GLANDS; HUMAN CHROMOSOMES; HYBRIDIZATION; MAPPING; ORGANS; 550400* - Genetics

Citation Formats

Flejter, W J, Glover, T W, Barcroft, C L, Guo, Sun Wei, Boehnke, M, Chandrasekharappa, S, Collins, F S, Lynch, E D, Hayes, S, and Weber, B L. Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21. United States: N. p., 1993. Web. doi:10.1006/geno.1993.1382.
Flejter, W J, Glover, T W, Barcroft, C L, Guo, Sun Wei, Boehnke, M, Chandrasekharappa, S, Collins, F S, Lynch, E D, Hayes, S, & Weber, B L. Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21. United States. https://doi.org/10.1006/geno.1993.1382
Flejter, W J, Glover, T W, Barcroft, C L, Guo, Sun Wei, Boehnke, M, Chandrasekharappa, S, Collins, F S, Lynch, E D, Hayes, S, and Weber, B L. 1993. "Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21". United States. https://doi.org/10.1006/geno.1993.1382.
@article{osti_7160547,
title = {Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21},
author = {Flejter, W J and Glover, T W and Barcroft, C L and Guo, Sun Wei and Boehnke, M and Chandrasekharappa, S and Collins, F S and Lynch, E D and Hayes, S and Weber, B L},
abstractNote = {A gene designated BRCA1, implicated in the susceptibility to early-onset familial breast cancer, has recently been localized to chromosome 17q12-q21. To date, the order of DNA markers mapped within this region has been based on genetic linkage analysis. The authors report the use of multicolor fluorescence in situ hybridization to establish a physically based map of five polymorphic DNA markers and 10 cloned genes spanning this region. Three cosmid clones and Alu-PCR-Generated products derived from 12 yeast artificial chromosome clones representing each of these markers were used in two-color mapping experiments to determine an initial proximity of markers relative to each other on metaphase chromosomes. Interphase mapping was then employed to determine the order and orientation of closely spaced loci by direct visualization of fluorescent signals following hybridization of three probes, each detected in a different color. Statistical analysis of the combined data suggests that the order of markers in the BRCA1 regions is cen-THRA1-TOP2-GAS-OF2-17HSD-248yg9-RNU2-OF3-PPY/p131-EPB3-Mfd188-WNT3-HOX2-GP3A-tel. This map is consistent with that determined by radiation-reduced hybrid mapping and will facilitate positional cloning strategies in efforts to isolate and characterize the BRCA1 gene. 27 refs., 2 figs., 3 tabs.},
doi = {10.1006/geno.1993.1382},
url = {https://www.osti.gov/biblio/7160547}, journal = {Genomics; (United States)},
issn = {0888-7543},
number = ,
volume = 17:3,
place = {United States},
year = {Wed Sep 01 00:00:00 EDT 1993},
month = {Wed Sep 01 00:00:00 EDT 1993}
}