Determination of the functional size of oxytocin receptors in plasma membranes from mammary gland and uterine myometrium of the rat by radiation inactivation

Journal Article · Sun May 01 00:00:00 EDT 1988 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-122-5-1769· OSTI ID:7154978

Soloff, M S; Beauregard, G; Potier, M

Gel filtration of detergent-solubilized oxytocin (OT) receptors in plasma membrane fractions from both regressed mammary gland and labor myometrium of the rat, showed that specific (/sup 3/H)OT binding was associated with a heterogeneously sized population of macromolecules. As radiation inactivation is the only method available to measure the apparent molecular weights of membrane proteins in situ, we used this approach to define the functional sizes of OT receptors. The results indicate that both mammary and myometrial receptors are uniform in size and of similar molecular mass. Mammary and myometrial receptors were estimated to be 57.5 +/- 3.8 (SD) and 58.8 +/- 1.6 kilodaltons, respectively. Knowledge of the functional size of OT receptors will be useful in studies involving the purification and characterization of the receptor and associated membrane components.

• Research Organization: Medical College of Ohio, Toledo (USA)
• OSTI ID: 7154978
• Journal Information: Endocrinology; (United States), Vol. 122:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MEMBRANE PROTEINS
MOLECULAR WEIGHT
OXYTOCIN
BIOCHEMICAL REACTION KINETICS
RECEPTORS
SIZE
CELL MEMBRANES
IRRADIATION
MAMMARY GLANDS
RATS
SOLUBILITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
ANIMALS
BODY
CELL CONSTITUENTS
FEMALE GENITALS
GLANDS
HORMONES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
560152 - Radiation Effects on Animals- Animals