Isolation of a photoactive photosynthetic reaction center - core antenna complex from Heliobacillus mobilis

Journal Article · Tue Dec 26 00:00:00 EST 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00452a003· OSTI ID:7152270

Trost, J T; Blankenship, R E ^[1]

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1. Arizona State Univ., Tempe (USA)

A photoactive reaction center-core antenna complex was isolated from the photosynthetic bacterium Heliobacillus mobilis by extraction of membranes with Deriphat 160c followed by differential centrifugation and sucrose density gradient ultracentrifugation. The purified complex contained a M{sub r} 47000 polypeptide(s) that bound both the primary donor (P800) and approximately 24 antenna bacteriochlorophylls g. Time-resolved fluorescence emission spectroscopy indicated that the antenna bacteriochlorophylls g are active in energy transfer to P800, exhibiting a decay time of 25 ps. The complex contained 1.4 menaquinones, 9 Fe, and 3 labile S{sup 2{minus}} per P800. The complex was photoactive with an exponential decay time of 14 ms for P800{sup +} yet showed no EPR-detectable Fe-S center signal in the g {le} 2.0 region, either by chemical reduction to -600 mV or by illumination of reduced samples. The complex is similar to photosystem I or oxygen-evolving photosynthetic systems in that both the primary donor and a core antenna are bound to the same pigment-protein complex.

• OSTI ID: 7152270
• Journal Information: Biochemistry; (USA), Vol. 28:26; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
PHOTOSYNTHETIC MEMBRANES
ABSORPTION SPECTRA
PHOTOSYNTHETIC REACTION CENTERS
MOLECULAR STRUCTURE
CHLOROPHYLL
ELECTRON SPECTRA
ELECTROPHORESIS
FLUORESCENCE SPECTROSCOPY
MOLECULAR WEIGHT
PHOTOSYNTHETIC BACTERIA
PURIFICATION
ULTRACENTRIFUGATION
CARBOXYLIC ACIDS
CENTRIFUGATION
EMISSION SPECTROSCOPY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)