Comparison of human and monkey cells for the ability to attenuate transcripts that begin at the adenovirus major late promoter

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7151167

Seiberg, M; Aloni, Y ^[1]; Levine, A J ^[2]

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1. Weizmann Institute of Science, Rehovot (Israel)
2. Princeton Univ., NJ (USA)

Late transcription from the adenovirus major late promoter can terminate prematurely at a site 182 to 188 nucleotides downstream. Experiments have been designed, with run-on transcription in nuclei in vitro or riboprobe protection of RNA obtained both in vivo and in vitro, that demonstrate that the ratio of attenuator RNA to readthrough RNA is greater in monkey cells (CV-1) than in human cells (HeLa). This may explain, in part, why the human adenoviruses replicate more poorly in CV-1 cells than in HeLa cells. A mutant adenovirus that replicates better than wild-type virus in monkey cells produces less of the attenuator RNA than wild-type adenovirus does in monkey cells. Monkey cell extracts have been shown to contain a factor that, when added to human cell extracts transcribing adenovirus DNA in vitro, increases the production of attenuator RNA in these reactions. These observations help to explain a portion of the block to the production of infectious adenoviruses in monkey cells.

• OSTI ID: 7151167
• Journal Information: Journal of Virology; (USA), Vol. 63:9; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
MOLECULAR BIOLOGY
GENE REPRESSORS
TRANSCRIPTION
ANIMAL CELLS
DNA HYBRIDIZATION
DNA SEQUENCING
HELA CELLS
MAN
MESSENGER-RNA
MONKEYS
PHOSPHORUS 32
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550701* - Microbiology- Tracer Techniques