Development of in vivo genotoxicity tests in estuarine fish and their application to aquatic toxicology
Genotoxicity assays were developed in embryonic stages of two species of fish, striped bass (Morone saxitilis) and sheepshead minnow (Cyprinodon variegatus), and in the adults of Fundulus heteroclitus using metaphase chromosome aberrations as the endpoint. Dose-dependent responses were obtained with several chemical mutagens, including 9-aminoacridine, ethylmethane sulphonate, cyclophosphamide and n-methyl-n-nitro-n-nitroguanidine, added to estuarine water at doses spanning several orders of magnitude in compound concentration. Exposures ranged from one to four days; however, two-day exposure time was found to be optimal in eggs and larvae. Tissues from the gills, kidney and intestinal tract of adult Fundulus were found to be responsive to mutagen exposure; however, the intestine gave the best responses. The results of these experiments suggest that these assays are sufficiently sensitive to be used in the field as well as in laboratory tests.
- Research Organization:
- Louisiana State Univ., Baton Rouge, LA (USA). Inst. for Environmental Studies
- OSTI ID:
- 7150143
- Report Number(s):
- PB-90-147695/XAB
- Resource Relation:
- Other Information: Pub. in Marine Environmental Research, Vol. 24, 327-331(1988)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACRIDINES
MUTAGEN SCREENING
EMS
FISHES
SENSITIVITY
NITRO COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
ESTUARIES
GASTROINTESTINAL TRACT
IN VIVO
KIDNEYS
LIVER
MUTAGENS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
AZINES
BODY
DIGESTIVE SYSTEM
ESTERS
GLANDS
HETEROCYCLIC COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PYRIDINES
SCREENING
SULFONIC ACID ESTERS
SURFACE WATERS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology