Sulfation of chondroitin. Specificity, degree of sulfation, and detergent effects with 4-sulfating and 6-sulfating microsomal systems

Journal Article · Tue Apr 05 00:00:00 EDT 1988 · J. Biol. Chem.; (United States)

OSTI ID:7143099

Sugumaran, G; Silbert, J E

Microsomal preparations from chondroitin 6-sulfate-producing chick embryo epiphyseal cartilage, and from chondroitin 4-sulfate-producing mouse mastocytoma cells, were incubated with UDP-(14C)glucuronic acid and UDP-N-acetylgalactosamine to form non-sulfated proteo(14C)chondroitin. Aliquots of the incubations were then incubated with 3'-phosphoadenylylphosphosulfate (PAPS) in the presence or absence of various detergents. In the absence of detergents, there was good sulfation of this endogenous proteo(14C)chondroitin by the original microsomes from both sources. Detergents, with the exception of Triton X-100, markedly inhibited sulfation in the mast cell system but not in the chick cartilage system. These results indicate that sulfation and polymerization are closely linked on cell membranes and that in some cases this organization can be disrupted by detergents. When aliquots of the original incubation were heat inactivated, and then reincubated with new microsomes from chick cartilage and/or mouse mastocytoma cells plus PAPS, there was no significant sulfation of this exogenous proteo(14C) chondroitin with either system unless Triton X-100 was added. Sulfation of exogenous chondroitin and chondroitin hexasaccharide was compared with sulfation of endogenous and exogenous proteo(14C)chondroitin. Sulfate incorporation into hexasaccharide and chondroitin decreased as their concentrations (based on uronic acid) approached that of the proteo(14C)chondroitin. At the same time, the degree of sulfation in percent of substituted hexosamine increased. However, the degree of sulfation did not reach that of the endogenous proteo(14C)chondroitin. Hexasaccharide and chondroitin sulfation were stimulated by the presence of Triton X-100. However, in contrast to the exogenous proteo(14C)chondroitin, there was some sulfation of hexasaccharide and chondroitin in the absence of this detergent.

• Research Organization: Veterans Administration Outpatient Clinic, Boston, MA (USA)
• OSTI ID: 7143099
• Journal Information: J. Biol. Chem.; (United States), Vol. 263:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHONDROITIN
METABOLISM
DETERGENTS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CHICKENS
GLUCURONIC ACID
MAST CELLS
MICE
MICROSOMES
SPECIFICITY
TRACER TECHNIQUES
TUMOR CELLS
ADDITIVES
ALDEHYDES
AMINES
ANIMAL CELLS
ANIMALS
BIRDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
EMULSIFIERS
FOWL
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MUCOPOLYSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
POLYSACCHARIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
SURFACTANTS
VERTEBRATES
WETTING AGENTS
550501* - Metabolism- Tracer Techniques