Human erythrocytes inhibit complement-mediated solubilization of immune complexes by human serum

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:7140877

Dorval, B L

The aim of this study was to develop an autologus human system to evaluate the effects of human erythrocytes on solubilization of immune complex precipitates (IC) by human serum. Incubation of IC with fresh human serum or guinea pig serum resulted in solubilization of IC. When packed erythrocytes were added to human serum or guinea pig serum binding of IC to the erythrocyte occurred and IC solubilization was inhibited significantly (p <.025). Sheep erythrocytes did not bind IC or inhibit IC solubilization. To evaluate the role of human erythrocyte complement receptor (CR1) on these findings, human erythrocytes were treated with trypsin or anti-CR1 antibodies. Both treatments abrogated IC binding to human erythrocytes but did not affect the ability of the human erythrocyte to inhibit IC solubilization. Radioimmunoassay was used to measure C3, C4 and C5 activation in human serum after incubation with IC, human erythrocytes, human erythrocytes plus IC, whole blood or in whole blood plus IC.

• Research Organization: Ohio State Univ., Columbus (USA)
• OSTI ID: 7140877
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
COMPLEMENT
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BIOLOGICAL FUNCTIONS
IMMUNE REACTIONS
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ANIMALS
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550201* - Biochemistry- Tracer Techniques