Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region
Poliovirus polysomal RNA is naturally uncapped, and as such, its translation must bypass any 5' cap-dependent ribosome recognition event. To elucidate the manner by which poliovirus mRNA is translated, the authors determined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. They found striking differences in translatability among the altered mRNAs when assayed in mock-infected and poliovirus-infected HeLa cell extracts. The results identify a functional cis-acting element within the 5' noncoding region of the poliovirus mRNA which enables it to translate in a cap-independent fashion. The major determinant of this element maps between nucleotides 320 and 631 of the 5' end of the poliovirus mRNA. They also show that this region (320 to 631), when fused to a heterologous mRNA, can function in cis to render the mRNA cap independent in translation.
- Research Organization:
- Dept. of Biochemistry and McGill Cancer Center, McGill Univ., Montreal, Quebec (CA)
- OSTI ID:
- 7131055
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:3
- Country of Publication:
- United States
- Language:
- English
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