Studies on RBC lipid and protein phosphorylation during blood bank storage

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7108743

Dumaswala, U J; Bryan, D J; Greenwalt, T J

Recent evidence has suggested that phosphoinositides play a significant role in maintaining membrane structure and function. Their importance during blood bank storage is not understood. They have performed preliminary studies of the phosphoinositide synthetic pathway enzymes of RBC during blood bank storage. At 0 and 35 days of storage leaky ghosts were prepared and incubated with (..gamma..-/sup 32/P)ATP for 5 minutes at 30 C. One aliquot was subjected to acidified solvent extraction and thin layer chromatography. The labeled phosphoinositide -4,5 biphosphate (PIP/sub 2/), phosphoinositide-4 phosphate (PIP) and phosphatidic acid (PA) spots were scraped and counted by liquid scintillation spectrometry. Another aliquot was used for SDS-PAGE and the radioactivity associated with the ..beta..-spectrin was measured. These experiments suggest a decrease in RBC phosphoinositol and PIP-Kinases and ..beta..-spectrin kinase activities during blood bank storage. Further studies are being done to evaluate significance of these observations.

• Research Organization: Univ. of Cincinnati, OH
• OSTI ID: 7108743
• Report Number(s): CONF-8606151-; TRN: 86-039035
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD
STORAGE
PHOSPHOLIPIDS
PHOSPHORYLATION
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROTEINS
ATP
BIOLOGICAL PATHWAYS
ELECTROPHORESIS
ERYTHROCYTES
PHOSPHORUS 32
SOLVENT EXTRACTION
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERS
EXTRACTION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MATERIALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
SEPARATION PROCESSES
TRANSFERASES
550201* - Biochemistry- Tracer Techniques