Effect of diabetes and insulin on the turnover of hexokinase II in the skeletal muscle
An ELISA procedure was developed to determine the amount of hexokinase II protein in the skeletal muscle extracts of rats, and immunoprecipitation was utilized to determine the hexokinase II activity. Both hexokinase II activity and the amount of hexokinase II protein decreased in the diabetic rat. Both increased toward normal treatment with insulin. The rate of synthesis of hexokinase II in the skeletal muscle was determined by the rate of incorporation of (/sup 3/H) leucine into hexokinase II. This rate was compared to that of the total cytosolic proteins to determine if the rate of hexokinase II synthesis was specifically affected by insulin. This relative rate of synthesis of hexokinase II was found to be approximately two times greater in the normal as compared to the diabetic rat. The apparent rate constants of degradation were determined using a double-isotope technique and from these constants it was possible to calculate the apparent half-lives. The apparent half-life was approximately 2.3 times greater in the normal compared to the diabetic rat and 2 times greater in the insulin-treated compared to the diabetic.
- Research Organization:
- Iowa State Univ. of Science and Technology, Ames (USA)
- OSTI ID:
- 7108337
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEXOKINASE
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
ENZYME ACTIVITY
DIABETES MELLITUS
DOUBLE LABELLING
ENZYME IMMUNOASSAY
EXPERIMENTAL DATA
HALF-LIFE
INSULIN
LABELLED COMPOUNDS
LEUCINE
MUSCLES
RATS
RESPONSE MODIFYING FACTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
DATA
DISEASES
ENDOCRINE DISEASES
ENZYMES
HORMONES
IMMUNOASSAY
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLING
MAMMALS
METABOLIC DISEASES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
REACTION KINETICS
RODENTS
SYNTHESIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques