Endotoxin-induced mortality in rats is reduced by nitrones

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Circulatory Shock; (USA)

OSTI ID:7104742

Hamburger, S A; McCay, P B ^[1]

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1. Oklahoma Medical Researh Foundation, Oklahoma City (USA)

The goal of these investigations was to determine if nitrone spin-trapping agents can alter mortality associated with endotoxemia in the rat. Reactive free radicals attack nitrone spin-trapping agents forming relatively reactive, persistent free radical spin adducts. We administered 85 mM (10 ml/kg) of alpha-phenyl N-tert-butyl nitrone (PBN), alpha-4-pyridyl-N-oxide N-tert-butyl nitrone (4-POBN), 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), or vehicle (saline i.p.) 30 min before endotoxin (25 mg/kg i.p.) or vehicle to Sprague-Dawley (SD) or Holtzman virus-free (HVF) rats (n = 10-17/group). All vehicle-treated rats receiving endotoxin were dead by 1 day. At 7 days, 83% of PBN-treated SD, 42% of PBN- or POBN-treated HVF, and 25% of DMPO-treated HVF rats were alive. The difference in survival of PBN-treated animals between strains may reflect the higher susceptibility of HVF rats to endotoxin. The observed reduction in mortality may be related to the well-established capacity of spin-trapping agents to capture reactive free radicals that may be generated in target tissues in response to endotoxin, and that would otherwise react with cell components and produce tissue injury.

• OSTI ID: 7104742
• Journal Information: Circulatory Shock; (USA), Vol. 29:4; ISSN 0092-6213
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
NITROGEN OXIDES
BIOLOGICAL EFFECTS
RADICALS
BIOSYNTHESIS
ENDOTOXINS
LIPOPOLYSACCHARIDES
MORTALITY
RATS
ANIMALS
ANTIGENS
CARBOHYDRATES
CHALCOGENIDES
LIPIDS
MAMMALS
MATERIALS
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
POLYSACCHARIDES
RODENTS
SACCHARIDES
SYNTHESIS
TOXIC MATERIALS
TOXINS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology