Response of sheep lymphocytes to PHA: quantitation by nuclear volume measurement and cell counts (40764)

Journal Article · Sat Mar 01 00:00:00 EST 1980 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-163-40764· OSTI ID:7099567

Chandra, P; Chanana, A D; Joel, D D

Phytohemagglutinin response of peripheral blood lymphocytes (PBL) of sheep was studied. Assessment of proliferative response was performed by determination of nuclear volumes and cell counts in cultures from 14 sheep and by incorporation of tritiated thymidine in cultures in four additional sheep. PBL of sheep were found to transform and proliferate with PHA similarly to human peripheral blood lymphocytes with minor differences. Quantitation of the proliferative response by determining the cell count and nuclear volumes provided more information on cell kinetics in culture than the commonly used isotope-labeled thymidine incorporation method.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 7099567
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Vol. 163:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL NUCLEI
VOLUME
LYMPHOCYTES
STIMULATION
CELL CULTURES
CELL PROLIFERATION
PHYTOHEMAGGLUTININ
SHEEP
AGGLUTININS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
HEMAGGLUTININS
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
RUMINANTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques