Hydrodynamic properties of solubilized atrial natriuretic factor receptor from bovine adrenal cortex
The authors have previously reported the pharmacological characterization of specific receptors for atrial natriuretic factor (ANF) in bovine adrenal cortex. In this study they report the physicochemical characteristics of this receptor solubilized with the nonionic detergent octyl glucoside. /sup 125/I-ANF binding activity was assayed by a PEG precipitation technique. Analysis of competitive binding curves with the soluble receptor preparation revealed the presence of high-affinity binding sites with a K/sub d/ of 40 pM and a density of 400 fmol/mg protein. The hydrodynamic properties of the solubilized receptor prelabeled with /sup 125/I-ANF were then determined. The receptor-detergent complex eluted as a major peak with a stokes radius of 51.3 A as determined by gel filtration on Superose-6. The sedimentation coefficient, S/sub 20 w/, of the complex was 6.19 S as determined by ultracentrifugation on a 5-20% sucrose gradient. From these data, the molecular weight of the ANF receptor-octyl glucoside complex was estimated to be 133,000 assuming a partial specific volume of 0.730 ml/g. This value is in agreement with the values that they have previously reported by SDS-gel electrophoresis.
- Research Organization:
- Clinical Research Institute of Montreal, Quebec
- OSTI ID:
- 7092957
- Report Number(s):
- CONF-8604222-; TRN: 87-008610
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques