Characterization of hormone-sensitive Madin-Darby canine kidney cells

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09101-7· OSTI ID:7092496

Lin, M C; Beckner, S K; Darfler, F J

The paper describes the optimal conditions for maintaining hormone responsiveness, measurement of intracellular AMP, and the characteristics of several types of hormone sensitivity in Madin-Darby canine kidney cells. Cyclic AMP is measured by radioimmunoassay with (/sup 175/I) as tracer. The responsiveness of the kidney cells to glucagon, vasopressin, isoproterenol, and prostaglandin in presented.

• OSTI ID: 7092496
• Journal Information: Methods Enzymol.; (United States), Vol. 109
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMP
RADIOIMMUNOASSAY
ANIMAL CELLS
BIOLOGICAL FUNCTIONS
KIDNEYS
BIOCHEMICAL REACTION KINETICS
DOGS
EXPERIMENTAL DATA
GLUCAGON
IODINE 125
LABELLED COMPOUNDS
PROSTAGLANDINS
RESPONSE MODIFYING FACTORS
SENSITIVITY
SYMPATHOMIMETICS
VASOPRESSIN
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
DATA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NUCLEI
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PITUITARY HORMONES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques