Diffusion behavior of lysozyme in aqueous ammonium sulfate solutions under varying solution conditions as determined by dynamic light scattering

Technical Report · Thu Dec 01 00:00:00 EST 1994

OSTI ID:7092350

Fornefeld, U M; Kuehner, D E; Blanch, H W; Prausnitz, J M ^[1]

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1. Univ. of California, Berkeley, CA (United States). Dept. of Chemical Engineering Lawrence Berkeley Lab., CA (United States). Chemical Sciences Div.

As proteins gain significance in commercial applications such as pharmaceuticals, detergents, organic waste management and cosmetics, efficient and economical recovery of these valuable biomolecules is of increasing importance. the salting-out process has found widespread application in the area of protein separations. To date, salt-induced precipitation of proteins from complex aqueous solutions remains largely an empirical process; no comprehensive model exists to predict salting-out phase equilibria in protein solutions. Rational predictive models for salt-induced precipitation will therefore be of great value in protein purification, both on the preparative and the analytical scale. Any attempt to model theoretically salt-induced protein precipitation must include the known physics of protein interactions in aqueous solution. With this in mind, it is crucial to acknowledge that protein precipitation is fundamentally an aggregation process. In order to incorporate aggregation effects into ongoing efforts to model salting out of proteins, it is necessary to quantify the degree of aggregation as a function of solution conditions. Therefore, dynamic light scattering measurements were performed with a well-studied protein, hen-egg-white lysozyme, under several solution conditions.

• Research Organization: Lawrence Berkeley Lab., CA (United States)
• Sponsoring Organization: USDOE; National Science Foundation (NSF); USDOE, Washington, DC (United States); National Science Foundation, Washington, DC (United States)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 7092350
• Report Number(s): LBL-36510; ON: DE95004821
• Country of Publication: United States
• Language: English

Related Subjects

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
59 BASIC BIOLOGICAL SCIENCES
LYSOZYME
AGGLOMERATION
DIFFUSION
ORGANIC COMPOUNDS
WASTE PROCESSING
EXPERIMENTAL DATA
FORECASTING
MATHEMATICAL MODELS
DATA
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
MANAGEMENT
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
PROCESSING
PROTEINS
WASTE MANAGEMENT
320305* - Energy Conservation
Consumption
& Utilization- Industrial & Agricultural Processes- Industrial Waste Management
550200 - Biochemistry