Decrease in IgE Fc receptor expression on mouse bone marrow-derived mast cells and inhibition of PAF-acether formation and of. beta. -hexosaminidase release by dexamethasone
The effect of dexamethasone (DM) on the immunologic and nonimmunologic release of paf-acether and of the granule marker ..beta..-hexosaminidase (BHEX) from mouse bone marrow-derived mast cells (BMMC) was studied. Preincubation of BMMC with DM inhibited in a dose-dependent fashion the immunologic release of paf-acether and of BHEX as compared with control cells. The antigen-induced increase in acetyltransferase activity, used as an index of cellular activation, was inhibited by 37 +/- 16% in 1 ..mu..M DM-treated BMMC as compared with untreated cells. Preincubation of BMMC with DM for 24 hr caused a dose-dependent inhibition of /sup 125/I-IgE binding to the cells, with a half-maximal effect at 14 nM. The number of IgE Fc receptors was decreased by 55% in 1 ..mu..M DM-treated BMMC as compared with untreated cells. Cytofluorometer analysis of BMMC sensitized with a saturating amount of purified monoclonal IgE, followed by addition of a fluoresceinated anti-mouse IgG (heavy and light chains), revealed a single cellular population for both DM-treated and untreated BMMC. The possible link between the decreased sensitization of the cells consequent to the reduction in IgG Fc receptor expression and the alteration of the secretory response and acetyltransferase activity was investigated. BMMC were incubated with IgE under experimental conditions giving half-sensitization of the cells. Upon antigen challenge, a 10.5 +/- 3.7% decrease in acetyltransferase activity and a 29.2 +/- 3.5% decrease in paf-acether release were observed with half-sensitized cells as compared with cells sensitized with a saturating amount of IgE. These results indicate that DM inhibits the immunologic release of paf-acether and of BHEX from passively sensitized BMMC and decreases the IgE Fc receptor number available for sensitization. Thus, the modulation of IgE Fc receptor number could explain part of the anti-allergic properties of glucocorticosteroids.
- Research Organization:
- INSERM, Clamart, France
- OSTI ID:
- 7092190
- Journal Information:
- J. Immunol.; (United States), Vol. 136:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DEXAMETHASONE
BIOLOGICAL EFFECTS
IMMUNOGLOBULINS
RECEPTORS
ALLERGY
AMIDINASES
BIOCHEMICAL REACTION KINETICS
BONE MARROW CELLS
CELL FLOW SYSTEMS
CONCANAVALIN
CORTICOSTEROIDS
GLUCOCORTICOIDS
IODINE 125
LABELLED COMPOUNDS
LYMPHOCYTES
MAST CELLS
MICE
RADIOIMMUNOLOGY
RADIORECEPTOR ASSAY
ADRENAL HORMONES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLOBULINS
HEMAGGLUTININS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LECTINS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
NON-PEPTIDE C-N HYDROLASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PREGNANES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES
550301* - Cytology- Tracer Techniques