Postirradiation recovery of lymphoid cells in the rat
Whole-body irradiation has been extensively used to remove immune responsiveness in rodent recipients in adoptive allograft assays. This study was undertaken to determine the relative radioresistance and the tempo of regeneration, following whole-body irradiation, of cells involved in the allograft response. Six distinct cell populations have been identified in the lymphoid tissues of rats subjected to sublethal whole-body irradiation. The relative representation of these subpopulations was significantly different from that in nonirradiated controls. NK cells, macrophages, and plasma cells, which are present in very low numbers in cell suspensions prepared from normal lymphoid tissues, made up a significant proportion of the residual/regenerating population in the tissues of rats recovering from whole-body irradiation. More significantly perhaps, the mature T cell populations showed a significant increase in the T cytotoxic/suppressor to T helper cell ratio. These observations support the suggestion that a number of the cell types within the mixed cell population observed in the rejecting indicator grafts of irradiated recipients in adoptive allograft assays are host derived. The finding that the T cytotoxic/suppressor population is apparently more radioresistant than the T helper population supports a conclusion that graft rejection in irradiated recipients, restored with pure populations of T helper cells, may not be directly mediated by the injected cells but may be the result of collaboration between these and host-derived cytotoxic cell populations.
- Research Organization:
- Univ. of Sydney, New South Wales (Australia)
- OSTI ID:
- 7091490
- Journal Information:
- Transplantation; (United States), Vol. 46:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNITY
RADIOINDUCTION
LYMPHOCYTES
CELL PROLIFERATION
IMMUNE REACTIONS
LYMPH NODES
RATS
SPLEEN
SUBLETHAL IRRADIATION
THYMECTOMY
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MEDICINE
ORGANS
RODENTS
SOMATIC CELLS
SURGERY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals